thyB [2017-8-11 16:0:3]

thymidylate synthase B

locus: BSU21820

pI: 6.27

mw: 30.39 kDa

protein length: 264 aa

gene length: 792 bp

function: biosynthesis of thymidine nucleotides

product: thymidylate synthase B

essential: no

ec: 2.1.1.45

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.5|Nucleotide metabolism] → [category|SW 2.5.2|Biosynthesis/ acquisition of nucleotides] → [category|SW 2.5.2.4|Biosynthesis/ acquisition of pyrimidine nucleotides]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.1|Phosphorylation on an Arg residue]

Gene

Coordinates: 2,297,106 → 2,297,900

The protein

Catalyzed reaction/ biological activity

• 5,10-methylenetetrahydrofolate dUMP = dihydrofolate dTMP (according to Swiss-Prot)

Paralogous protein(s)

• [protein|6FE1D3C4D4DF86DE2D3D9A143A1DCFE120F13F5F|ThyA]

Modification

• phosphorylated on Arg-102 [Pubmed|22517742]

Structure

• [PDB|3IX6] (from ''Brucella melitensis'', 67% identity)

Expression and Regulation

Operons

• genes: [gene|8B515C789439354F870023E69077D2C1D709E046|ypjP]-[gene|2CB3BAF17F59A858D249619A436EF660CAA58DD5|ypjQ]-[gene|9F9643D51E9ABA537892B19312491697CECDDCCF|thyB]-[gene|B740E971204AC14BA456B8C93A86D4BDC29524FF|dfrA]-[gene|9D1DAA5E69DD12FDE0FB33EA0863E0CB214120EC|ypkP]
  description: [Pubmed|22383849]

• genes: [gene|9F9643D51E9ABA537892B19312491697CECDDCCF|thyB]-[gene|B740E971204AC14BA456B8C93A86D4BDC29524FF|dfrA]
  description: [Pubmed|2840350]

  regulatory mechanism

  • [protein|2234D81F8F9B92EAF1CF6F73BEC92D4DE54E2636|CcpN]: activation, weak, in [regulon|2234D81F8F9B92EAF1CF6F73BEC92D4DE54E2636|CcpN regulon]

  regulation

  • weak induction in the presence of glucose ([protein|search|CcpN]) [Pubmed|19732150]

References

Reviews

• 7574499

Original publications

• 418407,19732150,2840350,22517742